7/29/2023 0 Comments Ecl solution western blot![]() Pierce ECL Western Blotting Substrate provides performance identical to the original Amersham ECL Western Blotting Detection Reagent from GE Healthcare. It enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods.īecause the luminol and peroxide reagent formulations are identical to other commercially available substrate products, one can switch to Pierce ECL without needing to optimize probing conditions or incubation protocols. WesternBright ECL is more sensitive than other chemiluminescent substrates and uses up to 10 times less antibody than other substrates, allowing you to save precious antibodies and samples. ![]() The high sensitivity ECL substrate produces a strong signal with very low. It uses an enhanced chemiluminescent substrate for western blotting that was developed for film imaging and is also compatible with CCD imaging. No optimization required easy to switch from other entry-level ECL substrates. WesternBright ECL is a horseradish peroxidase substrate optimized for chemiluminescent Western blots imaged using X-ray film. High Sensitivity ECL Substrate Kit ab133406 is designed for the detection of proteins with 23pg-187ng of protein per band. Pierce ECL Western Blotting Substrate provides reliability and performance equivalent to other standard ECL substrates for detection of HRP enzyme activity. The ECL Western Blotting Substrate detects and visualizes the presence of picogram (pg) amounts of antigen through the use of photographic or other suitable chemiluminescent imaging methods. Recommended secondary antibody concentration: 1:1,000–1:15,000 dilution (0.07–1.0 µg/mL)Ĭompare and learn more about our western blot chemiluminescent substrates ›.Compatibility: nitrocellulose and PVDF membranes.Stability: 1-hr working solution stability, 1-year kit stability at 4☌.Pierce ECL Plus Western Blotting Substrate characteristics include: Thermo Scientific Pierce ECL Western Blotting Substrate is a value-priced, entry-level horseradish peroxidase (HRP) substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.
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